Spring Antibody Update

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Spring Antibody Update

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AFP Ab-2

(Rabbit Polyclonal)
Specificity and Comments: NEOMARKERS' polyclonal antibody recognizes an oncofetal glycoprotein with a single chain of 70kDa which is identified as alpha fetoprotein (AFP). This PAb is highly specific to AFP and shows no cross-reaction with other onco-fetal antigens. AFP is normally synthesized in the liver, intestinal tract, and yolk sac of the fetus. Antibody to AFP has been shown to be useful in detecting hepatocellular carcinomas (HCC) and germ cell neoplasms, especially yolk sac tumors. is excellent for immuno-histochemical staining of formalin-fixed, paraffin-embedded tissues.
Species Reactivity: Human, Pig, Sheep. Does not react with dog.
Immunogen: Purified alpha fetoprotein
Known Applications: Immunohistochemistry (frozen & formalin/paraffin)
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Alpha 1-antichymotrypsin Ab-1

(Rabbit Polyclonal)
Specificity and Comments: Recognizes a protein of 62kDa which is identified as Alpha-1-Antichymotrypsin (Alpha-1-ACT). This polyclonal antibody is highly specific to Alpha-1-ACT and shows no cross-reaction with other related proteins. Alpha 1-ACT is an early-stage acute-phase plasma protein and a serpin that preferentially inactivates chymotrypsin, cathepsin G, and chymase. Alpha-1-ACT, a serine protease inhibitor, is tightly associated with amyloid plaques in Alzheimer's disease (AD) and in normal aged human and monkey brain. Regulation of the serine proteases and serine protease inhibitors play an important role in neuromuscular differentiation. Prostate specific antigen (PSA), a chymotrypsin-like serine protease, is predominantly complexed to Alpha-1-ACT. A higher proportion of serum PSA is complexed to Alpha-1-ACT in prostate cancer than in benign prostate hyperplasia.
Immunogen: Purified human serum Alpha-1-Antichymotrypsin
Known Applications: Immunohistochemistry (frozen & formalin/paraffin)
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Alpha 1-antitrypsin Ab-1

(Rabbit Polyclonal)
Specificity and Comments: Shown to be human specific, this antibody is useful in for immunohistochemistry in frozen and paraffin sections.
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CA19-9/Sialyl Lewis^aAb-1

(Mouse Monoclonal 121SLE)
Specificity and Comments: This monoclonal is cited as working in ELISA's, and immunohistochemistry (frozen and formalin/paraffin sections). Human reactive, other species have not been tested.
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Cathepsin D Ab-1

(Mouse Monoclonal C5)
Specificity and Comments: Cathepsin D is synthesized as a 54kDa precursor which is proteolytically processed to an intermediate 48kDa single chain which matures into more stable 34kDa and 14kDa two chain form. It is an estrogen-regulated lysosomal protease that has been suggested to facilitate cancer cell migration and invasion by digesting the basement membrane, extracellular matrix, and connective tissue. Because of its mitogenic and proteolytic activities, it has been implicated as a prognostic marker in many tumor types, especially breast cancer. Cathepsin D is expressed in epithelial cells as well as in macrophages. Immunoreactivity of cancer cells and tissue infiltrating macrophages must be recorded separately. This MAb is superb for immuno-histochemical staining of formalin-fixed, paraffin-embedded tissues.
Species Reactivity: Human; Others-not tested
Immunogen: Cathepsin D purified from human spleen
Known Applications: Immunohistochemistry (Frozen & Formalin/paraffin)
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CD3 Ab-1

(Rabbit Polyclonal)
Specificity and Comments: Shown to be human reactive, this antibody is useful in for immunohistochemistry in frozen and paraffin sections. Other species have not been tested.
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CD3 Ab-2

(Mouse Monoclonal PS1)
Specificity and Comments: This monoclonal is cited as working in Western blotting, and immunohistochemistry (frozen and formalin/paraffin sections). Human reactive, other species have not been tested.
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CD5 Ab-1

(Mouse Monoclonal 4C7)
Specificity and Comments: Recognizes a 67kDa transmembrane protein which is identified as CD5. The CD5 antigen is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by many T cell leukemia, lymphomas, and activated T cells. Occasionally, CD5 antigen is also expressed on a subset of B cells. Mantle cell lymphomas (same as diffuse centrocytic lymphomas) are CD5+ while the follicle center cell lymphoma are CD5-. This MAb is superb for identifying the formalin-fixed, paraffin-embedded mantle cell lymphomas.
Species Reactivity: Human; Others-not tested
Immunogen: Recombinant protein corresponding to the external domain of the CD5 molecule
Known Applications: Western Blotting, Immunohistochemistry (Formalin/paraffin)
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CD6 Ab-1

(Mouse Monoclonal SPV-L14)
Specificity and Comments: Recognizes a 120-130kDa glycoprotein which is identified as CD6. It is homologous to CD5 and macrophage scavenger receptor. It is expressed on the surface of mature thymocytes, peripheral T cells, and a subset of B cells. CD6 may be involved in T-cell activation as MAb’s to CD6 augment T-cell receptor (TCR)-mediated activation of T cells, and the CD6 molecule is tyrosine phosphorylated during TCR-mediated T-cell triggering. NEOMARKERS' SPV-L14 MAb is superb for immunohistochemical staining of formalin/paraffin tissues.
Species Reactivity: Human; Others-not tested
Immunogen: CD8+ cytotoxic T-cell clone.
Known Applications: Inhibits CD3 MAb-induced Proliferation of PBL. Does Not Inhibit PHA-induced Proliferation of PBL. Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistology (frozen and formalin/paraffin)
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CD15 Ab-1

(Mouse Monoclonal BRA-4F1)
Specificity and comments: Shown to be human reactive, this antibody is useful in for immunohistochemistry in frozen and paraffin sections. Other species have not been tested.
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CD19/B-Cell Ab-1

(Mouse Monoclonal)
Specificity and Comments: Recognizes a 96kDa single chain glycoprotein which is identified as CD19. This protein is present on the surface of human B-cells. CD19 is important for detecting both normal and neoplastic B-cells. Because of its broad spectrum of reactivity within the B-cell lineage, NEOMARKERS' FMC63 MAb detects neoplasms arising from early B-cells (e.g. acute leukemia of pre-B-cells). It also reacts with the majority of more differentiated B-cell neoplasms (e.g. chronic lymphocytic leukemia and non-Hodgkin’s lymphoma). However, it does not react with neoplastic plasma cells in myelomas and plasmocytomas.
Species Reactivity: Human; Others-not tested
Immunogen: Raji Cells.
Known Applications: Flow Cytometry, Immunofluorescence, Immunohistology (frozen)
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CD20/B-Cell Ab-1

(Mouse Monoclonal L26)
Specificity and Comments: Recognizes a protein of 30-33kDa which is identified as CD20. L26 MAb reacts with the majority of B-cells present in peripheral blood and lymphoid tissues and their derived lymphomas. In lymphoid tissue, germinal center blasts and B immunoblasts are particularly reactive with L26. This MAb is shown to be a reliable antibody for ascribing a B-cell phenotype in known lymphoid tissues. Rarely, L26-positive T-cell lymphomas have been reported. Reactivity has also been noted with Reed-Sternberg cells in cases of Hodgkin’s disease, particularly of lymphocyte predominant type.
Species Reactivity: Human, Baboon, Monkey. Does not react with cow, dog, pig, rat. Others-not tested
Immunogen: Human tonsil B cells
Known Applications: Flow Cytometry, Immunofluorescence, Immunohistology (frozen and formalin/paraffin)
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CD20/B-Cell Ab-2

(Mouse Monoclonal B9E9)
Specificity and Comments: This monoclonal is cited as working in imnunofluorescence, flow cytometry, and immunohistochemistry (frozen sections). Human reactive, other species have not been tested.
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CD30 Ab-1

(Mouse Monoclonal Ber-H2)
Specificity and Comments: Shown to be human reactive, this antibody is useful in for immunohistochemistry in frozen and paraffin sections. Other species have not been tested.
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CD31/Endothelial Cell Ab-1

(Mouse Monoclonal JC/70A)
Specificity and Comments: Recognizes a 100kDa glycoprotein in endothelial cells and 130kDa in platelets. This MAb reacts with endothelial cells in normal tissues and in benign and malignant proliferations. In cryostat sections and blood smears the antibody also stains megakaryocytes, platelets and occasionally plasma cells. It reacts weakly with mantle zone B cells, peripheral T cells, and neutrophils. Antibody to CD31 is of value in the study of benign and malignant vascular tumors. Staining for CD31 has also been used to measure angiogenesis which reportedly predicts tumor recurrence.
Species Reactivity: Human; does not react with pig; Others-not tested
Immunogen: Membrane preparation of a spleen from a patient with hairy cell leukemia
Known Applications: Immunofluorescence, Western Blotting, Immunohistology (frozen and formalin/paraffin)
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CD34 Ab-1

(Mouse Monoclonal QBEnd/10)
Specificity and Comments: Recognizes a single chain transmembrane glycoprotein of 110kDa which is identified as CD34. This antigen is selectively expressed on human lymphoid and myeloid hematopoietic progenitor cells. The antibody to CD34 also reacts with vascular endothelial cells in normal tissues and in benign and malignant proliferations. This antibody is of value in the study of benign and malignant vascular tumors as well as characterization of leukemias. Staining for CD34 has been used to measure angiogenesis which reportedly predicts tumor recurrence.
Species Reactivity: Human; Others-not tested
Immunogen: Detergent solubilized vesicular suspension prepared from a perfusate of human term placenta
Known Applications: Flow Cytometry, Immunohistology (frozen and formalin/paraffin)
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CD35/CR1 Ab-2

(Mouse Monoclonal E11)
Specificity and Comments: Recognizes a protein of 210-220kDa which is identified as the complement receptor 1(CR1)/CD35. This antigen is present on a subpopulation of T lymphocytes (~5%) which are relatively low in CD3 expression, on all CD20-positive B lymphocytes and CD14-positive monocytes, and on poly-morphonuclear leukocytes (PMNs). The primary function of CR1 is to serve as the cellular receptor for C3b and C4b, the most important components of the complement system leading to clearance of foreign macromolecules. NEOMARKERS' E11 MAb is excellent for immunohistochemical staining of formalin/paraffin tissues.
Species Reactivity: Human; Others-not tested
Immunogen: Intact human cells of monocyte lineage
Known Applications: Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blotting, Immunohistology (frozen and formalin/paraffin)
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CD45/LCA Ab-2

(Mouse Monoclonal PD7/26/16+2B11)
Specificity and Comments: Recognizes the CD45 leukocyte common antigen (LCA) family which is comprised of at least four isoforms of membrane glycoproteins (220, 205, 190, 180kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portion of these molecules have protein phosphatase activity and are involved in regulation of transmembrane signals. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
Species Reactivity: Human; does not react with dog; Others-not tested
Immunogen: PD7/26/16: human peripheral blood lymphocytes maintained in T cell growth factor. 2B11: isolated neoplastic cells from T cell lymphoma.
Known Applications: Flow Cytometry, Immunofluorescence, Immunohistology (frozen and formalin/paraffin)
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CD45/LCA Ab-3

(Mouse Monoclonal Cocktail)
Specificity and Comments: Recognizes the CD45 leucocyte common antigen (LCA) family which is comprised of at least four isoforms of membrane glycoproteins (220, 205, 190, 180kDa) expressed on hematopoietic cell lines but absent on non-hematopoietic cell lines, normal and malignant non-hematopoietic tissues. The intracellular portion of these molecules have protein phosphatase activity and are involved in regulation of transmembrane signals. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.
Species Reactivity: Human; Others-not tested
Known Applications: ELISA, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blotting, Immunohistochemistry (frozen & formalin/paraffin)
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CD45RB Ab-1

(Mouse Monoclonal BRA-11)
Specificity and Comments: This monoclonal is cited as working in imnunofluorescence, flow cytometry, and immunohistochemistry (frozen and formalin/paraffin sections). Human reactive, other species have not been tested.
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CD55/DAF Ab-1

(Mouse Monoclonal)
Specificity and Comments: This monoclonal is cited as working in imnunofluorescence, flow cytometry, and immunohistochemistry (frozen and formalin/paraffin sections). Human reactive, other species have not been tested.
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CD68/Macrophage Ab-1

(Mouse Monoclonal KP1)
Specificity and Comments: Recognizes a glycoprotein of 110kDa which is identified as CD68. KP1 MAb is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. KP1 reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Tumors of lymphoid origin are usually not stained.
Species Reactivity: Human, Monkey, Cat; does not react with Pig, Dog, chicken
Immunogen: Subcellular fraction of human alveolar macrophages
Known Applications: Immunohistology (frozen and formalin/paraffin)
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CD79a/B-Cell Ab-1

(Mouse Monoclonal HM47/A9)
Specificity and Comments: A disulphide-linked heterodimer, consisting of mb-1 and B29 polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B cell Ag receptor. Two components of this complex are designated CD79a and CD79b respectively. The Ag CD79a, or mb-1 polypeptide, first appears at pre B cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. The antigen CD79a is found in the majority of acute leukemia's of precursor B cell type, in B cell lines, B cell lymphomas, and in some myelomas. It is not present in myeloid or T cell lines.
Species Reactivity: Human, Monkey, Horse, Cow, Pig, Rabbit, Guinea pig, Rat, Mouse; Chicken (Plasma cells)
Immunogen: Synthetic peptide consisting of GTYQDVGSLNIADVQ of human CD79a protein.
Known Applications: Immunofluorescence, Western Blotting, Immunohistology (frozen and formalin/paraffin)
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CD87/UPA-Receptor Ab-1

(Mouse Monoclonal 10G7)
Specificity and Comments: This monoclonal is cited as working in imnunofluorescence, flow cytometry, and immunohistochemistry (frozen sections). Human reactive, other species have not been tested.
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cdk6 Ab-1

(Mouse Monoclonal K6.83)
Specificity and Comments: Recognizes a protein of 40kDa, identified as p40 or cyclin-dependent kinase 6 (p40cdk6 also known as PLSTIRE). MAb K6.83 is highly specific to p40cdk6 an applications including staining of formalin-fixed, paraffin-embedded tissues.
Species Reactivity: Human; Others-not tested
Immunogen: Purified recombinant cdk6 protein
Known Applications: Immunofluorescence, Immunoprecipitation (co-precipitates Cyclin D’s), Kinase Assay , Western Blotting, Immunohistology (frozen; formalin/paraffin)
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cdk6 Ab-2

(Mouse Monoclonal K6.90)
Specificity and Comments: Recognizes a protein of 40kDa, identified as p40 or cyclin-dependent kinase 6 (p40cdk6 also known as PLSTIRE). MAb K6.90 is highly specific to p40cdk6 and shows no cross-reaction with other cdks. A family of proteins designated as cdks are critical regulators of cell cycle progression. The prototype member of this family, p34cdc2, and a related protein cdk2, function late in the cycle while cdk4 and cdk6 are critically involved in G1 to S progression. NEOMARKERS' K6.90 MAb is excellent for multiple applications including staining of formalin-fixed, paraffin-embedded tissues.
Species Reactivity: Human; Others-not tested
Immunogen: Purified recombinant cdk6 protein
Known Applications: Immunofluorescence, Immunoprecipitation (co-precipitates Cyclin D’s), Kinase Assay, Western Blotting, Immunohistology (frozen; formalin/paraffin)
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CEA Ab-1

(Mouse Monoclonal C260)
Specificity and Comments: It recognizes a glycoprotein of 180kDa, identified as carcinoembryonic antigen (CEA), which is synthesized during development in the fetal gut, and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. NEOMARKERS' C260 MAb reacts with specific regions on CEA and shows no cross-reactivity with neutrophils. Antibody to CEA is reportedly useful in identifying the origin of various metastatic adenocarcinomas and in distinguishing pulmonary adenocarcinomas (60-70% are CEA+) from pleural mesotheliomas (rarely or weakly CEA+).
Species Reactivity: Human; Others-not tested
Immunogen: Human tumor
Known Applications: ELISA, Immunohistology (frozen & formalin/paraffin)
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CEA Ab-2

(Rabbit Polyclonal)
Specificity and Comments: Shown to be human reactive, this antibody is useful in for immunohistochemistry in paraffin sections. Other species have not been tested.
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Chromogranin A Ab-1

(Mouse Monoclonal LK2H10)
Specificity and Comments: Recognizes a protein of 68-75kDa, identified as chromogranin A (a protein of 439-amino acid which is encoded on chromosome 14). Epitopes of NEOMARKERS' LK2H10 and PHE5 MAbs are different. Chromogranin A is present in neuro-endocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheo-chromocytomas, paragangliomas, and other neuro-endocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.
Species Reactivity: Human, Monkey, Pig. Does not react with Sheep, Rabbit, Mouse, Rat, Guinea pig
Immunogen: Human pheochromocytoma
Known Applications: Immunohistology (frozen and formalin/paraffin)
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Chromogranin A Ab-2

(Mouse Monoclonal PHE5)
Specificity and Comments: Recognizes a protein of 68-75kDa, identified as chromogranin A (a protein of 439-amino acid which is encoded on chromosome 14). Epitopes of NEOMARKERS' LK2H10 and PHE5 MAbs are different. Chromogranin A is present in neuro-endocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheo-chromocytomas, paragangliomas, and other neuro-endocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.
Species Reactivity: Human; Others-not tested
Immunogen: Human pheochromocytoma
Known Applications: Immunofluorescence, Immunohistology (frozen and Carnoy or formalin-fixed/paraffin)
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Chromogranin A Ab-3

(Mouse Monoclonal LK2H10+PHE5)
Specificity and Comments: Recognizes a protein of 68-75kDa, identified as chromogranin A (a protein of 439-amino acid which is encoded on chromosome 14). Epitopes of NEOMARKERS' Ab-1 (LK2H10) and Ab-2 (PHE5) are different. Ab-3 (a cocktail of Ab-1 and Ab-2) is especially designed for sensitive detection of human Chromogranin A in formalin-fixed, paraffin-embedded tissues. Chromogranin A is present in neuroendocrine cells throughout the body, including the neuroendocrine cells of the large and small intestine, adrenal medulla and pancreatic islets. It is an excellent marker for carcinoid tumors, pheo-chromocytomas, paragangliomas, and other neuro-endocrine tumors. Coexpression of chromogranin A and neuron specific enolase (NSE) is common in neuroendocrine neoplasms.
Species Reactivity: Human
Immunogen: Human pheochromocytoma
Known Applications: Immunohistology (frozen and formalin/paraffin)
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CNPase (Oligodendrocyte) Ab-1

(Mouse Monoclonal 11-5B)
Specificity and Comments: This MAb recognizes Human, Murine, Cat, Pig, Cow, and Sheep CNPase. This antibody is known to work in immunofluorescence, immunohistochemistry (frozen and formalin/paraffin sections), Immunoprecipitation, and Western blotting.
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Collagen IV Ab-1

(Mouse Monoclonal PHM-12)
Specificity and Comments: This MAb is highly specific to type IV collagen and shows no cross-reaction with other types of collagens. Collagen IV is a major constituent of the basement membranes. In kidney, PHM-12 MAb reacts with glomerular and tubular basement membranes, parts of mesengial matrix and the Bowman’s capsule. It also reacts with basal lamina of capillaries as well as basement membranes in a variety of tissues. Antibody to collagen IV is useful in detecting the loss of parts of basement membrane in carcinomas.
Species Reactivity: Human; Others-not tested
Known Applications: Immunohistochemistry (frozen & formalin/paraffin)
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Collagen IX Ab-1

(Mouse Monoclonal 2C2)
Specificity and Comments: This MAb is highly specific to type IX collagen and shows no cross-reaction with types I, II, III, IV, V, or X. Type IX collagen-proteoglycan is a major component of hyaline cartilages where it is located on the surface of the collagen fibrils so that a collagenous domain of the molecule (called COL3) and a non-collagenous domain (called NC4) project at periodic distances away from the surface of the fibrils. Type IX collagen-proteoglycan is also present on the surface of the collagen fibrils of the adult chicken vitreous but, unlike cartilage, lacks the NC4 domain and possesses a very long chondroitin sulfate chain which provides an extensive coat to the fibril. NEOMARKERS' Ab-1 recognizes an epitope close to the C-terminus (COL2) of the HMW fragment, which is present in both the long- and short-form type IX collagens, whereas Ab-2 recognizes an epitope in the N-terminal COL3 (NC4) domain of the long-form type IX collagen, which is absent in the short-form. Ab-1 recognizes pepsin-resistant type IX collagen fragments from both cartilage and vitreous whereas Ab-2 only recognizes the fragments from cartilage. In embryonic chicks, Ab-1-reactive type IX collagen is found within the notochord at stage 14 and in the notochordal sheath at stage 20.
Species Reactivity: Chicken, Quail. Non-reactive with human, rat and guinea pig. Others-not tested
Known Applications: Electron Microscopy, ELISA, Immunohistology (frozen), Immunofluorescence (digest with hyaluronidase), Rotary Shadowing, Western Blotting
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Collagen IX Ab-2

(Mouse Monoclonal 4D6)
Specificity and Comments: This MAb is highly specific to type IX collagen and shows no cross-reaction with types I, II, III, IV, V, or X. Type IX collagen-proteoglycan is a major component of hyaline cartilages where it is located on the surface of the collagen fibrils so that a collagenous domain of the molecule (called COL3) and a non-collagenous domain (called NC4) project at periodic distances away from the surface of the fibrils. Type IX collagen-proteoglycan is also present on the surface of the collagen fibrils of the adult chicken vitreous but, unlike cartilage, lacks the NC4 domain and possesses a very long chondroitin sulfate chain which provides an extensive coat to the fibril. NEOMARKERS' Ab-1 recognizes an epitope close to the C-terminus (COL2) of the HMW fragment, which is present in both the long- and short-form type IX collagens, whereas Ab-2 recognizes an epitope in the N-terminal COL3 (NC4) domain of the long-form type IX collagen, which is absent in the short-form. Ab-1 recognizes pepsin-resistant type IX collagen fragments from both cartilage and vitreous whereas Ab-2 only recognizes the fragments from cartilage. In embryonic chicks, Ab-2 reactive type IX collagen is not found within the notochord nor in the notochordal sheath.
Species Reactivity: Chicken, Quail. Non-reactive with human, rat and guinea pig. Others-not tested
Immunogen: Pepsin resistant fragments of type IX collagen from an acid extract of lathyritic chicken cartilage (called HMW and LMW)
Known Applications: Electron Microscopy, ELISA, Immunofluorescence (digest with hyaluronidase), Immunohistology (frozen), Rotary Shadowing, Western Blotting
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Cyclin A Ab-1

(Mouse Monoclonal E23)
Specificity and Comments: It recognizes a protein of 60kDa which is identified as cyclin A. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk’s) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk’s. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase. NEOMARKERS' E23 MAb is extremely sensitive in detecting cyclin A by Western blotting.
Species Reactivity: Human, Cow, Mouse, Rat; Others-not tested
Known Applications: Immunoprecipitation, Western Blotting, Immunohistology (Frozen)
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Cyclin A Ab-2

(Mouse Monoclonal E72)
Specificity and Comments: It recognizes a protein of 60kDa which is identified as cyclin A. Cyclins are regulatory subunits of the cyclin-dependent kinases (cdk’s) and they control transition at different specific phases of the cell cycle. The temporal expression of cyclins is tightly regulated and subsequently plays a critical role in controlling the enzymatic activity of cdk’s. These cyclin/cdk complexes are essential for passage through specific stages in the cell cycle. In mammalian somatic cells, cyclin A is required for S-phase and passage through G2-phase. The D and E type cyclins regulate the passage of G1, while cyclin B is a critical regulator of mitosis. Mutation or disruption of normal cyclin A expression causes cells to arrest in G2-phase. NEOMARKERS' E72 MAb supports the kinase activity and co-precipitates cdk/Cyclin A H1 kinase/CIP-1 in an active trimeric complex.
Species Reactivity: Human, Cow, Mink, Mouse, Rat; Others-not tested
Immunogen: Bovine Cyclin A
Known Applications: Immunoprecipitation, Kinase Assay, Immunohistology (Frozen)
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Cyclin B1 Ab-1

(Mouse Monoclonal V152)
Specificity and Comments: It recognizes a protein of 62kDa which is identified as cyclin B1. In mammals, cyclin B associates with inactive p34cdc2 which facilitates phosphorylation of p34cdc2 at aa 14Thr and 15Tyr. This maintains the inactive state until the end of G2-phase. The inactive cyclin B-p34cdc2 complex continues to accumulate in the cytoplasm until the completion of DNA synthesis, when Cdc25, a specific protein phosphatase, dephosphorylates aa 14Thr and 15Tyr of p34cdc2 rendering the complex active at the G2/M boundary. This mitotic kinase complex remains active until the metaphase/anaphase transition when cyclin B is degraded. This degradation process is ubiquitin-dependent and is necessary for the cell to exit mitosis. Therefore, cyclin B-p34cdc2 plays a critical role in G2 to M transition.
Species Reactivity: Human, Hamster; Others-not tested
Immunogen: Hamster Cyclin B1
Known Applications: Flow Cytometry, Immunoprecipitation (Inquire) Western Blotting, Immunohistology (frozen and formalin/paraffin)
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Desmin Ab-1

(Mouse Monoclonal D33)
Specificity and Comments: NEOMARKERS' D33 MAb recognizes a 53kDa intermediate filament protein which is identified as desmin. This MAb is highly specific to desmin and shows no cross-reaction with other intermediate filament proteins. Antibody to desmin reacts with striated (skeletal and cardiac) as well as smooth muscle cells. In skeletal and cardiac muscles, the staining is confined to the Z-bands giving a characteristic striated appearance. Anti-desmin antibody is useful in identification of tumors of myogenic origin. It reacts with leiomyosarcomas (smooth muscle) as well as rhabdomyosarcomas (striated muscle).
Species Reactivity: Human, Baboon, Monkey, Cow, Cat, Dog, Hamster, Rat, Chicken
Immunogen: Desmin purified from human muscle
Known Applications: Immunofluorescence, Immunohistology (frozen and formalin/paraffin)
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DNA-PK Ab-1

(Mouse Monoclonal 18-2)
Specificity and Comments: Recognizes a protein of 460kDa, identified as the catalytic subunit of DNA-dependent Protein Kinase (DNA-PKcs). The epitope for NEOMARKERS' Ab-1 maps in the N-terminal 2/3rd of DNA-PKcs. Although the epitopes for Ab-1, Ab-2, and Ab-3 are not precisely mapped, experimental data suggest that they are different. Ab-1 inhibits (~50%) the kinase activity of DNA-PKcs. However, preincubation of the enzyme with DNA protects DNA-PKcs against inactivation by Ab-1. DNA-PK phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. In vivo, it is involved in repairing double strand DNA breaks. The Ku autoantigen, a heterodimer of p70/p80 proteins, is important for the function of DNA-PK.
Species Reactivity: Human; Others-not tested. A cocktail of Ab-1, Ab-2, and Ab-3 has been successfully used to detect DNA-PK of Mouse, Hamster, Xenopus, & Chicken origin.
Immunogen: Human DNA-dependent Protein Kinase purified from HeLa cells
Known Applications: Gel Supershift (Use NEOMARKERS' Ab-4), Inhibits (~50%) DNA-dependent Phosphorylation, Immunofluorescence, Immunoprecipitation, Western Blotting
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DNA-PK Ab-2

(Mouse Monoclonal 25-4)
Specificity and Comments: Recognizes a protein of 460kDa, identified as the catalytic subunit of DNA-dependent Protein Kinase (DNA-PKcs). The epitope for NEOMARKERS' Ab-2 maps in the C-terminal 1/3rd of DNA-PKcs. Although the epitopes for Ab-1, Ab-2, and Ab-3 are not precisely mapped, experimental data suggest that they are different. DNA-PK phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. In vivo, it is involved in repairing double strand DNA breaks. The Ku autoantigen, a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins, is important for the function of DNA-PK.
Species Reactivity: Human; Others-not tested. A cocktail of Ab-1, Ab-2, and Ab-3 has been successfully used to detect DNA-PK of Mouse, Hamster, Xenopus, & Chicken origin.
Immunogen: Human DNA-dependent Protein Kinase purified from HeLa cells
Known Applications: Gel Supershift (Use NEOMARKERS' Ab-4), Immunofluorescence, Immunoprecipitation, Western Blotting
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DNA-PK Ab-3

(Mouse Monoclonal 42-psc)
Specificity and Comments: Recognizes a protein of 460kDa, identified as the catalytic subunit of DNA-dependent Protein Kinase (DNA-PKcs). The epitope for NEOMARKERS' Ab-3 maps in the C-terminal 2/3rd of DNA-PKcs. Although the epitopes for Ab-1, Ab-2, and Ab-3 are not precisely mapped, experimental data suggest that they are different. DNA-PK phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. In vivo, it is involved in repairing double strand DNA breaks. The Ku autoantigen, a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins, is important for the function of DNA-PK.
Known Species Reactivity: Human; Others-not tested. A cocktail of Ab-1, Ab-2, and Ab-3 has been successfully used to detect DNA-PK of Mouse, Hamster, Xenopus, & Chicken origin.
Clone Designation: 42-psc (also referred to as 42-26 or 42-27)
Immunogen: Human DNA-dependent Protein Kinase purified from HeLa cells
Known Applications: Gel Supershift (Use NEOMARKERS' Ab-4), Immunofluorescence, Immunoprecipitation Western Blotting
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DNA-PK Ab-4

(Mouse Monoclonal Cocktail)
Specificity and Comments: Recognizes a protein of 460kDa, identified as the catalytic subunit of DNA-dependent Protein Kinase (DNA-PKcs). The epitope for NEOMARKERS' Ab-3 maps in the C-terminal 2/3rd of DNA-PKcs. Although the epitopes for Ab-1, Ab-2, and Ab-3 are not precisely mapped, experimental data suggest that they are different. DNA-PK phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. In vivo, it is involved in repairing double strand DNA breaks. The Ku autoantigen, a heterodimer of 70kDa (p70) and ~80kDa (p80) proteins, is important for the function of DNA-PK.
Species Reactivity: Human; Others-not tested. A cocktail of Ab-1, Ab-2, and Ab-3 has been successfully used to detect DNA-PK of Mouse, Hamster, Xenopus, & Chicken origin.
Clone Designation: 42-psc (also referred to as 42-26 or 42-27)
Immunogen: Human DNA-dependent Protein Kinase purified from HeLa cells
Known Applications: Gel Supershift (Use NEOMARKERS' Ab-4), Immunofluorescence, Immunoprecipitation Western Blotting
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E-Cadherin Ab-2

(Rat monoclonal DECMA-1)
Specificity and Comments: It recognizes a set of 120, 102, and 92kDa proteins as recognized by other polyclonal and monoclonal antibodies against uvomorulin/E-cadherin.
Species Reactivity: Human, Mouse, Dog
Immunogen: Mouse embryonal carcinoma PCC4aza1 cells followed by a partially purified 84kDa trypsin digestion product of E-cadherin/uvomorulin from mouse F9 cells.
Known Applications: Blocks aggregation of mouse embryonal Ca cells & compaction of pre-implantation embryos (hence the name DECMA, Decompacting Monoclonal Antibody), Disrupts confluent monolayers of MDCK cells, ELISA, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blotting, Immunohistology (frozen & formalin/paraffin)
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EGF-R Ab-9

(Rabbit Polyclonal)
Specificity and Comments: It recognizes a 170kDa protein, identified as the first member (EGF-Receptor) of the type I family of growth factor receptors. EGF-R has an extracellular ligand binding domain, a single transmembrane region, and an intracellular domain. Epitope of Ab-9 maps in the cytoplasmic domain (aa984-996) of mouse EGF-R. Ab-9 is particularly well suited for Western blotting of mouse EGF-R. For Western blotting of human EGF-R, NEOMARKERS' Ab-7 is strongly recommended. EGF-R is widely expressed in mammals and has been implicated in various stages of embryonic development. EGF-R(-/-) mice survived up to 8 days after birth and suffered from impaired epithelial development in several organs, including skin, lung and gastrointestinal tract.
Species Reactivity: Human, Mouse; Others-not tested
Immunogen: A synthetic 13-mer peptide, corresponding to aa 984-996 from the mouse EGF-Receptor.
Known Applications: Western Blotting (Superb for mouse EGF-R). For Western Blotting of human EGF-R, use NEOMARKERS' Ab-12.
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EGF-R Ab-10

(Mouse Monoclonal 111.6)
Specificity and Comments: It recognizes a 170kDa protein, identified as the first member (EGF-Receptor) of type I family of growth factor receptors. Its epitope maps in the extracellular domain of EGF_r. It shows no cross reaction with erbB-2, erbB-3, or erbB-4. It blocks the binding of EGF to EGF-R. Overexpression of EGF-R has been reported in a variety of tumors including those of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium and indicates a poor prognosis. EGF-R is predominantly overexpressed in squamous cell carcinomas. NEOMARKERS' Ab-10 is superb for staining of routine formalin/paraffin tissues. Ab-12 is most sensitive for immunoprecipitation and Western blotting of human EGF-R.
Species Reactivity: Human; Others-not tested
Immunogen: Extracellular domain of human recombinant EGF-R protein
Known Applications: ELISA, Inhibits Binding of EGF to EGF-R, Immunoprecipitation of Unoccupied EGF-R, Western Blotting, Immunohistology (frozen & formalin/paraffin)
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EGF-R Ab-11

(Mouse Monoclonal 199.12)
Specificity and Comments: It recognizes a 170kDa protein, identified as the first member (EGF-Receptor) of type I family of growth factor receptors. Its epitope maps in the extracellular domain of EGF-R. It shows no cross reaction with erbB-2, erbB-3, or erbB-4. It does not block the binding of EGF to EGF-R. Overexpression of EGF-R has been reported in a variety of tumors including those of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium and indicates a poor prognosis. EGF-R is predominantly overexpressed in squamous cell carcinomas. NEOMARKERS' Ab-11 is excellent for Western blotting of extracellular domain of EGF-R.
Species Reactivity: Human; Others-not tested
Immunogen: Extracellular domain of recombinant human EGF-R protein
Known Applications: ELISA, Does Not Inhibit Binding of EGF to EGF-R, Immunoprecipitation of (Un)occupied EGF-R, Western Blotting, Immunohistology (Use NEOMARKERS' Ab-10)
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EGF-R Ab-12

(Mouse Monoclonal Cocktail R19/48)
Specificity and Comments: It recognizes a 170kDa protein, identified as the first member (EGF-Receptor) of type I family of growth factor receptors. Ab-12 recognizes the extracellular as well as the cytoplasmic domain of EGF-R. Overexpression of EGF-R has been reported in a variety of tumors including those of breast, brain, bladder, lung, gastric, head & neck, esophagus, cervix, vulva, ovary, and endometrium and indicates a poor prognosis. EGF-R is predominantly overexpressed in squamous cell carcinomas. NEOMARKERS' Ab-12 is specially designed for exquisite sensitive detection of EGF-R by immunoprecipitation and Western blotting.
Species Reactivity: Human; Others-not tested
Immunogen: Extracellular and cytoplasmic domains of recombinant human EGF-R protein
Known Applications: Immunoprecipitation, Western Blotting, Immunohistology (Use NEOMARKERS' Ab-10)
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EMA Ab-2

(Mouse Monoclonal GP1.4)
Specificity and Comments: In Western blotting, it recognizes two glycoproteins in MW range of 265-400kDa, identified as epithelial membrane antigen (EMA), or mucin 1 (MUC-1), or polymorphic epithelial mucin (PEM), or sialomucin, or episialin. NEOMARKERS' GP1.4 MAb reacts with the DTRP epitope in the tandem repeats. In immunohistochemical assays, it superbly stains routine formalin/paraffin carcinoma tissues without any special pretreatment. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Species Reactivity: Human; Others-not tested
Immunogen: Human milk fat globule membranes
Known Applications: ELISA, Immunoprecipitation, Western Blotting, Immunohistology (frozen & formalin/paraffin)
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Fibroblast Growth Factor Receptor Ab-1

(Mouse Monoclonal VBS6)
Specificity and Comments: This MAb recognizes Human FGF-R. This antibody is known to work in immunoprecipitation and Western blotting.
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Fibronectin Ab-4

(Mouse Monoclonal 568)
Specificity and Comments: Shown to be human reactive, this antibody is useful in for immunohistochemistry in frozen and paraffin sections. Other species have not been tested.
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Flk-1/VEGF-Receptor Ab-1

Flk-1/VEGF-Receptor Ab-2

(Mouse Monoclonal)
Specificity and Comments: Recognizes a protein of 200kDa, identified as Flk-1. Three cell membrane receptor tyrosine kinases, Flt, Flk-1, and Flt-4, putatively involved in the growth of endothelial cells, are characterized by the presence of seven immunoglobulin-like sequences in their extracellular domain. Three receptors exhibit high degree of sequence homology to each other as well as a lesser degree of relatedness to the class III receptors including CSF-1/Fms, PDGFR, SLFR/Kit, and Flt-3/Flk-2. Two members of this receptor class, Flt-1 and Flk-1, have been shown to represent high affinity receptors for vascular endothelial growth factors (VEGF). On the basis of structural similarity to Flt and Flk-1, it has been speculated that Flt-4 might represent a third receptor for either VEGF or a VEGF-related ligand.
Species Reactivity: Human; Others-not tested
Immunogen: Purified human recombinant Flk-1/VEGFR-2 protein
Known Applications: ELISA, Immunoprecipitation, Western Blotting, Immunohistology (Inquire)
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Fyn Ab-1

(Mouse Monoclonal 1S)
Specificity and Comments: Recognizes a protein of 59kDa which is identified as Fyn. p59fyn is a membrane-associated non-receptor protein tyrosine kinase that belongs to the Src-family of kinases. NEOMARKERS' 1S MAb shows no cross-reaction with other members of the Src protein kinase family. Its epitope spans between aa26-75 of the unique region of p59fyn. The unique N-terminal domain of p59fyn interacts with the CD3 and z chains of the TcR. p59fyn can bind other proteins (p82 and p116) through its SH2 and SH3 domains, which may act as substrates or regulators of fyn activity. p59fyn is highly expressed in brain suggesting that it may have a role in the sensory nervous network and in myelination at early stages of CNS formation.
Species Reactivity: Human, Mouse, Rat
Immunogen: Bacterial expressed GST-fusion protein construct corresponding to aa1-206 of murine p59fyn protein
Known Applications: ELISA, Immunohistology (Inquire), Immunoprecipitation, Kinase Assay, Western Blotting
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Heat Shock Factor I (HSFI) Ab-1

(Rat monoclonal 4B4)
Specificity and Comments: This MAb is reactive in Human, Monkey and Murine. This antibody is proven useful for immunoprecipitation and Western blotting. Other species have not been tested.
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Heat Shock Factor I (HSFI) Ab-2

(Rat monoclonal 10H4)
Specificity and Comments: This MAb is reactive in Human, Monkey and Murine. Other species have not been tested. This antibody is proven useful for immunoprecipitation and Western blotting.
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Heat Shock Factor I (HSFI) Ab-3

(Rat monoclonal 10H8)
Specificity and Comments: This MAb is reactive in Human, Monkey and Murine. This antibody is proven useful for immunoprecipitation and Western blotting. Other species have not been tested.
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Heat Shock Factor II (HSFII) Ab-1

(Rat monoclonal 3E2)
Specificity and Comments: This MAb is reactive in Monkey and Murine. Other species have not been tested. This antibody is proven useful for immunoprecipitation and Western blotting.
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HSP60 Ab-1

(Mouse Monoclonal LK1)
Specificity and Comments: Reacts with a protein of approximately 58kDa which is identified as hsp60. Use of human hsp60 deletion mutants suggests that its epitope maps either between amino acids 335-366 or 484-547. It shows no cross-reaction with human hsp70. High-power fluorescence microscopy of HEp-2 cells with this antibody reportedly revealed multiple small oval shapes distributed throughout the cytoplasm. This pattern is consistent with the described mitochondrial localization of hsp60.
Species Reactivity: Human
Immunogen: Purified hsp60 protein from human placenta.
Known Applications: Immunohistology (frozen and formalin/paraffin), Western Blotting
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HSP60 Ab-2

(Mouse Monoclonal LK2)
Specificity and Comments: Shown to react with human, murine, and bovine hsp60. Useful in ELISA, Electron Microscopy, Immunohistology (frozen and formalin/paraffin), Immunoprecipitation and Western blotting.
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HSP70 Ab-1

(Mouse Monoclonal BRM22)
Specificity and Comments: Recognizes both the constitutive (HSP73), and inducible (HSP72) forms of HSP70. A wide variety of environmental and pathophysiological stressful conditions trigger the synthesis of a family of proteins known as heat shock proteins (HSP’s), more appropriately called as stress response proteins (SRP’s). Many HSP’s, including members of the HSP70 family, are involved in processes such as protein denaturation-renaturation, folding-unfolding, transport-translocation, activation-inactivation, and secretion. HSP70 is found to be associated with steroid receptors, actin, p53, polyoma T antigen, nucleotides, and other unknown proteins. Also, HSP70 has been shown to be involved in protective roles against thermal stress, cytotoxic drugs, and other damaging conditions.
Species Reactivity: Human, Cow, Rabbit, Rat, Guinea pig, Chicken, Drosophila
Immunogen: Purified HSP70 from cow brain
Known Applications: ELISA, Immunofluorescence, Western Blotting, Immunohistology (frozen and formalin/paraffin)
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Involucrin Ab-1

(Mouse Monoclonal SY5)
Specificity and Comments: It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, SY5 reacts with a 120kDa protein. However, in other systems, it stains the involucrin in a variety of sizes: 170kDa in human breast cancer MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. The epitope of NEOMARKERS' SY5 MAb maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. The protein is a useful marker of terminal differentiation: in normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures it is expressed by all cells that have left the basal layer. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
Species Reactivity: Human, Gorilla, Owl monkey, Pig, Dog; does not react with mouse
Immunogen: Purified human involucrin
Known Applications: ELISA, Immunofluorescence, Immunoprecipitation, Western Blotting, Immunohistology (frozen and formalin/paraffin)
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Kappa Light Chain Ab-2

(Rabbit Polyclonal)
Specificity and Comments: This antibody reacts with Human, Monkey and several other species. It is useful for immunohistology (frozen and formalin/paraffin sections).
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Keratin 20 Ab-1

(Mouse Monoclonal Ks20.8)
Specificity and Comments: Recognizes an intermediate filament protein (IFP) of 46kDa which is identified as cytokeratin 20 (CK20). This MAb is highly specific to CK20 and shows no cross-reaction with other IFPs. CK20 is a Type-I keratin which is primarily expressed in gastric and intestinal epithelium, urothelium, and Merkel-cells. CK20 is expressed in adenocarcinomas of the colon, stomach, pancreas and the bile system. CK20 is also present in mucinous ovarian tumors, transitional-cell and Merkel-cell carcinomas. Notably, the squamous cell carcinomas and adenocarcinomas of the breast, lung, and endometrium, non-mucinous tumors of the ovary, and small cell carcinomas lack in CK20.
Species Reactivity: Human; Others-not tested
Immunogen: Semi-purified human cytokeratin preparation
Known Applications: Western Blotting, Immunohistology (frozen and formalin/paraffin)
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Keratin 5/6/18 Ab-1

(Mouse Monoclonal LP34)
Specificity and Comments: It recognizes polypeptides of 58kDa, 56kDa, and 45kDa, identified as keratin 5, 6, and 18 respectively. It shows no reaction with keratin 1, 8, or 19. LP34 MAb shows a broad pattern of reactivity with epithelial tissues, from simple glandular epithelia to stratified squamous epithelia. Epithelial cells are labelled whether they are ectodermal, mesodermal, or endodermal in origin. NEOMARKERS' LP34 MAb is useful in detecting cells of epithelial origin in mixed tumors, in effusions, in bone marrow samples, or in tissue culture cells.
Species Reactivity: Human, Cow, Rat
Immunogen: Detergent-insoluble fraction of psoriatic human epidermis
Known Applications: Immunohistochemistry (frozen & formalin/paraffin)
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Keratin, High Molecular Weight Ab-1

(Mouse Monoclonal AE3)
Specificity and Comments: Monoclonal antibody AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. Members of the acidic and basic subfamilies are found together in pairs. The composition of keratin pairs varies with the epithelial cell type, stage of differentiation, cellular growth environment, and disease state. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail which differentiates epithelial tumors from non-epithelial tumors.
Species Reactivity: Human; Others-not tested
Immunogen: Human epidermal Keratin
Known Applications: Immunofluorescence, Western Blotting, Immunohistology (frozen and formalin/paraffin)
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Keratin, High Molecular Weight Ab-2 (Like 34bE12)

(Mouse Monoclonal DE-SQ)
Specificity and Comments: It recognizes cytokeratin (CK) 13, 14, 15, and 16. Over 60 lung carcinomas were tested with DE-SQ MAb. All squamous carcinomas and adeno-squamous carcinomas were positively labeled whereas all adenocarcinomas were negative. In adenosquamous carcinomas, DE-SQ MAb stained only areas with the morphologic features of squamous differentiation. Sixty other randomly chosen squamous carcinomas from vulva, cervix, tongue, esophagus, anus, and floor of mouth were also positive with DE-SQ MAb, either totally or focally. In contrast, adenocarcinomas of colon, cervix, parotid gland, and rectum were invariably negative. In normal epithelia, DE-SQ positivity is confined to stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. NEOMARKERS' DE-SQ MAb promises to be a specific marker useful in differential diagnosis of squamous carcinomas from adenocarcinomas and differential diagnosis of benign and malignant tumors of prostatic gland.
Species Reactivity: Human; Others-not tested
Immunogen: Cytoskeletal fraction prepared from human gingiva by detergent/salt extraction.
Known Applications: Immunofluorescence,Western Blotting, Immunohistology (frozen and formalin/paraffin)
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Keratin, Low Molecular Weight Ab-1

(Mouse Monoclonal AE1)
Specificity and Comments: Monoclonal antibody AE1 recognizes the 56.5, 50, 50’, 48, and 40kDa keratins of the acidic subfamily. Twenty human keratins are resolved with two-dimensional gel electrophoresis into acidic (pI <5.7) and basic (pI >6.0) subfamilies. The acidic keratins have molecular weights of 56.5, 55, 51, 50, 50’, 48, 46, 45, and 40kDa. MAb AE3 recognizes the 65-67, 64, 59, 58, 56, and 52kDa keratins of basic subfamily. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail which differentiates epithelial tumors from non-epithelial tumors.
Species Reactivity: Human; Others-not tested
Immunogen: Human epidermal Keratin
Known Applications: Immunofluorescence, Western Blotting, Immunohistology (frozen and formalin/paraffin)
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Keratin, Pan Ab-1

(Mouse Monoclonal AE1+AE3)
Specificity and Comments: Monoclonal antibody AE1 recognizes the 56.5, 50, 50’, 48, and 40kDa keratins of acidic subfamily, whereas the AE3 MAb reacts with the basic keratins of 65-67, 64, 59, 58, 56, and 52kDa. AE1/AE3 reacts with keratinized (56.5/65-67) and corneal (55/64) epidermis, stratified squamous epithelia of internal organs (51/59), stratified epithelia (50/58), hyperproliferative keratinocytes (48/56), and simple epithelia (45/52 and 46/54). Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. AE1/AE3 is a broad spectrum anti pan-keratin antibody cocktail which differentiates epithelial tumors from non-epithelial tumors.
Species Reactivity: Human; Others-not tested
Immunogen: Human epidermal Keratin
Known Applications: Immunofluorescence, Western Blotting, Immunohistology (frozen and formalin/paraffin)
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Keratin, Pan Ab-2

(Mouse Monoclonal PAN-CK)
Specificity and Comments: PAN-CK is a broad spectrum anti pan-keratin antibody cocktail which recognizes keratins 4, 5, 6, 7, 8, 10, 13, 14, 15, 16, 18, and 19. Studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis. NEOMARKERS' PAN-CK cocktail differentiates epithelial tumors from non-epithelial tumors.
Species Reactivity: Human
Known Applications: Immunofluorescence, Western Blotting, Immunohistology (frozen and formalin/paraffin)
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Lambda Light Chain Ab-2

Lck (p56^lck) Ab-1

(Mouse Monoclonal 3A5)
Specificity and Comments:Recognizes a 56kDa protein identifiied as Lck. p56lck is a membrane-associated non-receptor protein tyrosine kinase that belongs to the Src-family of kinases. NEOMARKERS' 3A5 MAb shows no cross-reaction with other members of the Src protein kinase family. The unique N-terminal domain of p56lck interacts with the cytoplasmic tails of the CD4 and CD8 cell surface glycoproteins. Activated p56lck kinase can increase responsiveness of some T cell hybridomas to antigen. The phosphorylation status and the activity of p56lck is regulated by the CD45 tyrosine protein phosphatase. p56lck plays a critical role in T cell development and activation. In humans, p56lck gene is localized to a site in the genome which undergoes frequent chromosomal abnormalities in lymphomas and neuroblastomas.
Species Reactivity: Human, Mouse, Rat
Immunogen: Bacterial expressed GST-fusion protein construct corresponding to aa1-225 of murine p56lck protein
Known Applications: ELISA, Immunohistology (Inquire), Immunoprecipitation, Kinase Assay, Western Blotting
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Lysozyme (Muramidase) Ab-1

(Rabbit Polyclonal)
Specificity and Comments: This antibody reacts with a number of species, including Human, Cat, Monkey, Murine, Pig. Contact LVC for other information. Lysozyme Ab-1 is best suited for immunohistochemistry in formalin-fixed, paraffin-embedded sections.
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Melanoma Ab-1

(Mouse Monoclonal HMB45)
Specificity and Comments: By immunohistochemistry, it specifically recognizes a protein in melanocytes and melanomas. Biochemical nature of its antigen is yet not fully characterized. This MAb reacts with junctional and blue nevus cells and variably with fetal and neonatal melanocytes. Intradermal nevi, normal adult melanocytes, and non-melanocytic cells are negative. It does not stain tumor cells of epithelial, lymphoid, glial, or mesenchymal origin. HMB45 MAb is superb for identifying the formalin-fixed, paraffin-embedded melanomas and other tumors showing melanocytic differentiation.
Species Reactivity: Human; does not react with dog and rat. Others-not tested
Immunogen: Extract of pigmented melanoma metastases from lymph nodes.
Known Applications: Immunohistochemistry (Frozen & Formalin/paraffin)
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Mucin 3 (MUC3) Ab-1

(Mouse Monoclonal M3.1)
Specificity and Comments: This MAb is human reactive, and shown useful in immunohistology (frozen and formalin/paraffin sections). Other species not yet tested.
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Myeloperoxidase Ab-1

(Rabbit Polyclonal)
Specificity and Comments: This MAb is human reactive, and shown useful in immunohistology (frozen and formalin/paraffin sections). Other species not yet tested.
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MyoD1 Ab-1

(Mouse Monoclonal 5.8A)
Specificity and Comments: Recognizes a phosphoprotein of 45kDa, identified as MyoD1. The epitope of NEOMARKERS' Ab-1 maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. Ab-1 does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occassionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm’s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing’s sarcomas and alveolar soft part sarcomas.
Species Reactivity: Human, Mouse, Rat, Chicken
Immunogen: Recombinant mouse MyoD1 protein
Known Applications: Electron Microscopy, ELISA, Immunofluorescence, Immunoprecipitation, Western Blotting, Immunohistochemistry (frozen & formalin/paraffin)
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MyoD1 Ab-2

(Mouse Monoclonal 5.2F)
Specificity and Comments: Recognizes a phosphoprotein of 45kDa, identified as MyoD1. The epitope of NEOMARKERS' Ab-2 maps in between amino acid 3-56 in the N-terminus of mouse MyoD1 protein. Ab-2 does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occassionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm’s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing’s sarcomas and alveolar soft part sarcomas.
Species Reactivity: Human, Mouse, Rat, Chicken
Immunogen: Recombinant mouse MyoD1 protein
Known Applications: Electron Microscopy, ELISA, Immunofluorescence, Immunoprecipitation, Western Blotting, Immunohistochemistry (frozen & formalin/paraffin)
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Nerve Growth Factor Receptor Ab-1

(Mouse Monoclonal NGFR 5)
Specificity and Comments: Recognizes a glycoprotein of 75kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). Its epitope spans in aa1-160 of extracellular domain of NGFR/NTR. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75. In vitro, unbound p75NGFR promotes neural cell death, whereas, binding of p75NGFR by NGF ligand or antibody inhibits p75NGFR induced cell death. NEOMARKERS' NGFR5 MAb is superb for multiple applications including staining of formalin-fixed, paraffin-embedded tissues.
Species Reactivity: Human, Monkey, Baboon, Cat, Rabbit, Ferret; does not react with mouse and rat
Immunogen: NGFR from A875 melanoma cells
Known Applications: Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blotting (Non-reducing), Immunohistology (frozen and formalin/paraffin)
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Neurofilament Ab-1

(Mouse Monoclonal 2F11)
Specificity and Comments: Neurofilaments are the intermediate filaments of neurons and their processes. They are protein triplets composed of three major subunits of MW 68kDa, 180kDa, and 200kDa. NEOMARKERS' 2F11 MAb recognizes the phosphorylated form of 200kDa and 68kDa subunits. This Ab is highly specific and shows no cross-reaction with other intermediate filament proteins. 2F11 MAb labels neurons, neuronal processes, and peripheral nerves as well as sympathetic ganglion cells and adrenal medulla. Cell body of neurons, containing the non-phosphorylated neurofilament, is weakly stained. Neurofilaments are expressed in tumors of neural origin, or displaying neuronal differentiation, such as neuroblastoma, medulloblastoma, and retinoblastoma.
Species Reactivity: Human, Rabbit, Cat, Mouse, Rat. Does not react with dog. Others-not tested
Immunogen: Neurofilament purified from human brain
Known Applications: Immunofluorescence, Immunohistology (frozen and formalin/paraffin)
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NSE Ab-1

(Mouse Monoclonal E27)
Specificity and Comments: Recognizes a protein of 46kDa, identified as neuron-specific enolase (NSE). Enolases are homo- or heterodimers of the three subunits: alpha (46kDa), beta (44kDa), and gamma (46kDa). NEOMARKERS' E27 MAb shows no cross-reaction with the alpha- or beta-subunits of NSE. The alpha-subunit is expressed in most tissues and the beta-subunit only in muscle. The gamma-subunit is expressed primarily in neurons, in normal and in neoplastic neuroendocrine cells. Coexpression of NSE and chromogranin A is common in neuroendocrine neoplasms. NEOMARKERS' E27 MAb is superb for immunohistochemical staining of formalin-fixed, paraffin-embedded tissues.
Species Reactivity: Human; Others-not tested
Immunogen: Purified human neuron-specific enolase (NSE)
Known Applications: Western Blotting, Immunohistology (frozen and formalin/paraffin)
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p16^INK4a/MTS1 Ab-2

(Mouse Monoclonal ZJ11)
Specificity and Comments: Recognizes a 16kDa protein which is identified as the p16INK4a tumor suppressor protein. ZJ11 MAb is highly specific to p16INK4a and shows no cross-reaction with the closely related inhibitors such as p15INK4b and p18INK4c. p16INK4a is a specific inhibitor of cdk4 /cdk6, and a tumor suppressor involved in the pathogenesis of a variety of malignancies. NEOMARKERS' ZJ11 antibody is superb for immuno-histochemical staining of routine formalin/paraffin tissues.
Species Reactivity: Human; Others-not tested
Immunogen: Puified human recombinant full length p16 protein
Known Applications: Immunofluorescence, Immunoprecipitation, Western Blotting, Immunohistology (frozen and formalin/paraffin)
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p21^WAF1Ab-4

(Mouse Monoclonal)
Specificity and Comments: Shown to be human and murine reactive, this MAb is useful for immunoprecipitation.
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p21^WAF1 Ab-5

(Mouse Monoclonal)
Specificity and Comments: Shown to be human and murine reactive, this MAb is useful for Western blotting.
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p95^VAVAb-1

(Mouse Monoclonal VAV-30)
Specificity and Comments: Recognizes a 95kDa protein which is identified as the p95vav. NEOMARKERS' VAV-30 MAb is highly specific to p95vav, a proto-oncogene expressed in hematopoietic cells. p95vav contains a series of structural motifs found in intracellular signaling molecules and is tyrosine phosphorylated upon activation of hematopoietic cells through their surface receptors, such as the B cell IgM receptor complex or the T cell receptor-CD4 complex. It is also phosphorylated on serine and threonine. p95vav contains three SQ and one TQ sequences. Using the yeast two-hybrid system, it was shown that p70 Ku interacts with aa 813-837 of p95vav, part of the carboxyl-terminal Src homology 3 (SH3) domain. The interaction does not involve binding of the SH3 domain to a proline-rich domain of Ku. Instead, p95vav binds to aa 463-609 of p70 Ku, a region containing the DNA binding domain as well as leucine zipper-like motif. The leucine zipper (aa483-498) is not sufficient for the interaction with p95vav.
Species Reactivity: Human, Mouse, Rat; Others-not tested
Immunogen: Purified human recombinant full length p95vav-GST fusion protein
Known Applications: Immunofluorescence, Immunoprecipitation, Western Blotting
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Paxillin Ab-1

(Mouse Monoclonal 5H11)
Specificity and Comments: Shown to be human reactive, this MAb is useful for Immunofluorescence, Immunoprecipitation, and Western blotting.
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Phosphoinositol-3-Kinase (PI-3-K) Ab-1

(Mouse Monoclonal)
Specificity and Comments: Shown to be human reactive, this MAb is useful for Immunoprecipitation, and Western blotting. Other species not tested.
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Phospholipase Cd1 (PLCd1) Ab-1

PLAP Ab-2

(Rabbit Polyclonal)
Specificity and Comments: This PAb is human specific, and shown useful in immunohistology (frozen and formalin/paraffin sections).
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PLAP Ab-3

(Mouse Monoclonal H7E8)
Specificity and Comments: This MAb is human reactive, and shown useful in immunohistology (frozen and formalin/paraffin sections). Other species not tested.
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Renal Cell Carcinoma Ab-1

(Mouse Monoclonal PN-15)
Specificity and Comments: This MAb is human reactive, and shown useful in immunohistology (frozen and formalin/paraffin sections). Other species not tested.
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Retinol Binding Protein Ab-1

(Mouse Monoclonal G4E4)
Specificity and Comments: This MAb is human reactive, and shown useful in Immunohistology (frozen and formalin/paraffin sections), Immunoprecipitation, and Western blotting. Other species not tested.
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Synaptophysin Ab-1

(Rabbit Polyclonal)
Specificity and Comments: This MAb is human and dog reactive, and shown useful in immunohistology (frozen and formalin/paraffin sections). Other species not tested.
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Thrombospodin Ab-1

(Mouse Monoclonal A4.1)
Specificity and Comments: This MAb is human reactive, and shown useful in Biological Blockades, ELISA's, Immunoprecipitation, and Western blotting (native). Other species not tested.
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Thrombospondin Ab-2

(Mouse Monoclonal D4.6)
Specificity and Comments: This MAb is human reactive, and shown useful in ELISA's and Western blotting . Other species not tested.
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Thrombospondin Ab-3

(Mouse Monoclonal C6.7)
Specificity and Comments: This MAb is human reactive. It is cited as being useful in Biological Blockades, ELISA's, and Immunoprecipitation. Other species not tested.
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Thrombospondin Ab-4

(Mouse Monoclonal A6.1)
Specificity and Comments: This MAb is human reactive, and shown useful in Immunohistology (frozen and formalin/paraffin sections), ELISA's and Wesern blotting. Other species not tested.
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Thrombospondin Ab-5

(Mouse Monoclonal B5.2)
Specificity and Comments: This MAb is human reactive, and shown useful in Western blotting. Other species not tested.
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Toxoplasma Ab-1

(Rabbit Polyclonal)
Specificity and Comments: This antibody reacts with the protozoa toxoplasma gondii, an intracellular parasite. It is useful for immunohistology (frozen and formalin/paraffin sections).
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TNF alpha Ab-1

(Mouse Monoclonal J1D9)
Specificity and Comments: This MAb is human reactive. It is cited as being useful in Biological Blockades, ELISA's, Western blotting and Immunoprecipitation. Other species not tested.
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TNF alpha Ab-2

(Mouse Monoclonal J2D10)
Specificity and Comments: This MAb is human reactive. It is cited as being useful in Biological Blockades, ELISA's, Western blotting and Immunoprecipitation. Other species not tested.
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TNF alpha Ab-3

(Mouse Monoclonal C6-H6)
Specificity and Comments: Recognizes a protein of 17kDa which is identified as tumor necrosis factor alpha (TNF-a). Although the epitopes for NEOMARKERS' Ab-1, Ab-2, and Ab-3 are not precisely mapped, experimental data suggest that they are different. TNF-a is a macrophage/monocyte cytokine, usually found as a soluble secreted protein which has diverse immunomodulatory, anti-tumor, and toxic effects. NEOMARKERS' C6-H6 MAb is suitable for staining of formalin-fixed, paraffin-embedded tissues.
Species Reactivity: Human; Others-not tested.
Immunogen: A hexadecapeptide corresponding to aa115-130 of human TNF-a, conjugated to thyroglobulin
Known Applications: ELISA, Inhibits the Cytotoxic Effect of hrTNFa in vitro, Immunohistology (frozen and formalin/paraffin)
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VEGF Ab-3

(Mouse Monoclonal JH121)
Specificity and Comments: Recognizes proteins of 34-50kDa, identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). Multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues have been described which arise from alternative splicing of a single gene. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis which promotes tumor progression and metastasis. JH121 neutralizes the bioactivity of human and mouse VEGF.
Species Reactivity: Human, Mouse; Others-not tested
Immunogen: Purified human recombinant VEGF121 protein
Known Applications: Biological Blockade, ELISA, Immunoprecipitation, Western Blotting, Immunohistology(frozen & formalin/paraffin)
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VEGF Ab-4

(Mouse Monoclonal BFD31)
Specificity and Comments: Recognizes proteins of 34-50kDa, identified as various isoforms of Vascular Endothelial Growth Factor or Vascular Permeability Factor (VEGF/VPF). Multiple isoforms of VEGF containing 206-, 189-, 165-, and 121-amino acid residues have been described which arise from alternative splicing of a single gene. The smaller two isoforms, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two remain cell associated. VEGF/VPF plays an important role in angiogenesis which promotes tumor progression and metastasis. NEOMARKERS’ BFD31 MAb is excellent for multiple applications.
Species Reactivity: Human, does not react with mouse and rat; Others-not tested
Immunogen: A synthetic 20-mer peptide from the N-terminus of human VEGF protein.
Known Applications: ELISA, Immunoprecipitation, Western Blotting, Immunohistology(frozen & formalin/paraffin)
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